Rotating Live Mammalian Cells Free in Media Using Spatial Light Modulator (SLM)-Generated Optical Tweezers
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چکیده
منابع مشابه
Combined holographic-mechanical optical tweezers: Construction, optimisation and calibration
We have combined a spatial light modulator (SLM) and galvanometer-mounted mirrors into an optical tweezers set-up. This provides great flexibility by allowing us to create an array of traps which can be moved in a smooth and fast way. To optimise the performance we investigated the effect of incidence angle on the SLM with respect to phase and intensity response. Although it is possible to use ...
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We demonstrate a flexible setup for holographic steering of laser tweezers in microscopy using a high resolution spatial light modulator (SLM). In contrast to other methods, hologram read-out is done in the off-axis Fresnel regime rather than in the typically used on-axis Fourier regime. The diffractive structure is calculated as a Fresnel hologram, such that after reflection at the SLM only th...
متن کاملCombined holographic-mechanical optical tweezers: construction, optimization, and calibration.
A spatial light modulator (SLM) and a pair of galvanometer-mounted mirrors (GMM) were combined into an optical tweezers setup. This provides great flexibility as the SLM creates an array of traps, which can be moved smoothly and quickly with the GMM. To optimize performance, the effect of the incidence angle on the SLM with respect to phase and intensity response was investigated. Although it i...
متن کاملPositional stability of holographic optical traps.
The potential of digital holography for complex manipulation of micron-sized particles with optical tweezers has been clearly demonstrated. By contrast, its use in quantitative experiments has been rather limited, partly due to fluctuations introduced by the spatial light modulator (SLM) that displays the kinoforms. This is an important issue when high temporal or spatial stability is a concern...
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We present results of a correlation filter utilising a computer generated hologram using an analogue ferroelectric liquid crystal spatial light modulator (SLM). The SLM amplitude modulates light and can induce a 0/π phase shift, which is equivalent to modulating along the real axis. Two pixels are combined into a macropixel using a phase detour technique enabling full complex modulation. The me...
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تاریخ انتشار 2013